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Amazing Weekend

I think this past weekend was one of the best ever! On Friday I celebrated my 22nd birthday… I know, I'm old! I was planning on having a few friends come over and celebrate it with me. My roommates took me out to dinner, and when we returned to the apartment I opened the door and all my friends yelled "SURPRISE!" I was not expecting that at all. Everyone there played along by either telling me they weren't coming over or they couldn't come over till late. It was an awesome birthday! Saturday, I drove down to Richmond, Virginia to help celebrate a friend's 21st. They showed me around Richmond since I have never been there before which was pretty cool. It was great being able to hang out with these girls because I hardly get to see all of them together. On Sunday I had to get up bright and early to make the drive back to JMU in order to pick up my roommate and then we proceeded to drive to the Nissan Pavilion to see the Dave Matthews Band Concert. While up at the concert I met up with a couple of friends from freshman year which was pretty cool. The concert was absolutely amazing. It was a great way to end an incredible weekend.

But now it's the beginning of the week which means back to working in the lab. Yesterday, my lab partner and I spent most of our time ligating and transforming our cultures. After the transformation process, we streak out agar plates which are left in an incubator overnight so that the bacteria can grow. The plates are placed in the incubator in order to give the bacteria the most ideal environmental conditions for growing. Today, when my lab partner and I went into lab to check our cultures we found out that our plates came out negative, meaning there were no bacteria colonies present. We then had a decision to make. Because this was our second time in failing we had the choices of either starting completely over and re-PCRing our samples OR we could try to re-transform. We decided that it would probably be in our best interest to start completely over incase a mistake occured in the beginning steps of our experiment which might be the reason why we are failing in getting any bacterial colonies. Hopefully things go better for us this time around. Well I guess that's enough for now. It's gorgeous outside today so I'm going to the pool. Enjoy summer while it's here!

Posted by Kristen on June 28, 2005 09:34 AM | Link | Email Kristen